Translational_Unit

Part:BBa_K4099007:Design

Designed by: Nixue Song   Group: iGEM21_Shanghai_HS_ID   (2021-10-12)


sgRNA+HR+pNCas9 backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3475
    Illegal XbaI site found at 107
    Illegal XbaI site found at 286
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3475
    Illegal NheI site found at 670
    Illegal NheI site found at 3234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3475
    Illegal BglII site found at 104
    Illegal BglII site found at 2039
    Illegal BglII site found at 2093
    Illegal BamHI site found at 5513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3475
    Illegal XbaI site found at 107
    Illegal XbaI site found at 286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3475
    Illegal XbaI site found at 107
    Illegal XbaI site found at 286
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

No

References